Increased expression of Treg associated genes in enriched CD4+ T cells in females compared to males. The regulatory effects of CD4+ T cells are necessary to balance the inflammation and immune activation normally stimulated by CD4+ T cells. . FOXP3 and TGFβ expression are hallmarks of Regulatory T cells. Transcriptional analysis by Real-Time PCR indicates that (A) FOXP3 is expressed at higher levels in women compared to men as well as TGFβ (B). (* = p<.05). Increased number of FOX P3 positive cells were also observed by IHC with higher MFI in women compared to men (C, D, E) (Nucleus in blue, FOX P3 in Red).