Immunohistochemical analysis of jejunal mucosal samples. Tissue sections were stained for TNFα, IL17 and IL1β. A higher number of cells were observed that were positive for TNFα in women (3B) compared to men (3A). Quatitation for the mean fluorescence intensity of the tissue section showed similar results (3C). Women also had higher levels of IL17 + cells in the villus compared to men (3E vs 3D). On quantitation of mean fluorescence intensity within the villus, women had significantly higher levels compared to men (3F). IL1β staining in the jejunal mucosa in women was higher than in men (3G, H and I). No staining was observed in antibody negative controls. All images were obtained at a 63X magnification. (* = p<.05). Nucleus in blue; Antibody Positive cell in red.