Figure 3

Treatment with different estrogens reduces BMPR2 mRNA expression in multiple normal cell types. (A) BMPR2 expression by quantitative RT-PCR, normalized to HPRT, is reduced in cultured human lymphocytes treated with 1 μM E2 compared to untreated controls (P < 0.05). (B) BMPR2 expression by quantitative RT-PCR, normalized to HPRT, is reduced in cultured PMVECs treated with 1 μM E2 compared to untreated controls (P < 0.01). (C) BMPR2 expression by quantitative RT-PCR, normalized to HPRT, is reduced in cultured PMVECs treated with varying doses of E3 compared to untreated controls (P < 0.01 for each comparison). (D) Estrogen and induction of proliferation through PMA both independently inhibit BMPR2 expression, by densitometry of northern blot (three replicates per condition) at P < 0.05 by two-way ANOVA. The combination of proliferation and estrogen produces an approximately 4× inhibition of BMPR2 expression (P < 0.05 by Student's t test with adjustment for multiple comparisons).