Figure 5

Androgen receptor gene expression in intact and castrated males. Quantitative RT-PCR analysis of androgen receptor (AR) mRNA levels in intact and castrated males in the (a) left anterior descending coronary artery (LAD) (n = 9 for intacts, n = 7 for castrates) and (b) left ventricular microvessels (MV) (n = 10 for intacts, n = 8 for castrates). AR gene expression was normalized to GAPDH gene expression using the 2 ^-ΔΔCT method. Values are mean ± SEM.