Figure 8
Masking of the interfering effect of female miRNA preparation on kallikrein 1b26 (klk1b26) translation by specific single-strand DNA, [15th-44th]ssDNA. (A) Masking of the activity in female miRNA preparation interfering klk1b26 translation by [15th-44th]ssDNA ([15]). The sequences of single-strand DNAs, [15th-44th]ssDNA and [169th-198th]ssDNA ([169]) (used as negative control), corresponded with the 15th to 44th and 169th to 198th nucleotide positions of klk1b26 mRNA, respectively, and are described in Table 1. Prior to the addition into the in vitro translation system, the female miRNA preparation was incubated with either of the single-strand DNAs. The in vitro translation reaction and the [35S]klk1b26 protein analysis were carried out as described in Methods. Representative results are shown. Similar results were obtained from three independent experiments. (B) Quantitative determination of density of the [35S]klk1b26 protein band. Values represent the mean ± SD (n = 3) of the relative density.