Effects of castration and 5α-dihydrotestosterone (DHT) administration on the activity in the submandibular gland (SMG) miRNA preparation interfering kallikrein 1b26 (klk1b26) translation. (A) MicroRNAs were prepared as described in Methods from SMGs of castrated mouse, DHT-administered castrated mouse and DHT-administered female mouse, respectively. One miRNA sample was prepared from SMGs from one mouse. The effects of these miRNA preparations (90 ng/μl each) on klk1b26 translation were analyzed as described in the legend for Figure 4C. Representative results are shown. Similar results were obtained in three independent experiments. (B) Quantitative determination of density of the [35S]klk1b26 protein band by computer-assisted image analysis of the autoradiograms. Values represent the mean ± SD (n = 3 animals) of the relative density.