Reverse transcription (RT)-PCR for kallikrein 1b26 (klk1b26) mRNA by using various forward primers targeting the 5'-terminal region and middle region of the mRNA. (A) Positions of forward primers indicated the ordinal numbers of the nucleotides in the klk1b26 mRNA sequence (NM 010644) from its 5'-terminal end. Each forward primer was used in combination with the R552 reverse primer. Sequences of those primers are described in Table 1. (B, C) Total RNAs of ICR mouse submandibular glands (SMGs) were prepared from the following sources: male, castrated male (Cast), Cast + 5α-dihydrotestosterone (DHT), female, and female + DHT. (B) RT-PCR with primer pairs targeting the 5'-terminal region of klk1b26 mRNA was carried out by using either the primer pairs of F21 forward primer and R552 reverse primer or F21 forward primer and R609 reverse primer. (C) RT-PCR was carried out by using the F169 forward primer and R552 reverse primer or the F169 forward primer and R609 reverse primer targeting the middle region of klk1b26 mRNA. In (A-C), representative results are shown; similar results were obtained in three independent experiments.