Figure 2
From: Sex dependent regulation of osteoblast response to implant surface properties by systemic hormones
Only female cells respond to oestrogen when cultured on smooth and microstructured titanium (Ti) substrates. Osteoblasts from male and female rats were plated on Ti surfaces and treated with either E2 or E2 conjugated to bovine serum albumin (BSA; E2-BSA) at confluence. Cell number (A, B), alkaline phosphatase specific activity in cell lysates (C, D) and osteocalcin in the conditioned media (E, F) were measured after 24 hours. *P < 0.05, Ti surfaces versus tissue culture polystyrene; #P < 0.05, oestradiol versus control.