Localization of Foxl2 protein by immunofluorescence in the sex-changing gonad of three-spot wrasse. Red represents Foxl2 protein, whereas blue represents the nucleus. (A) Gonad at stage II. Degenerating oocyte (DO) is shown. (B) Section of gonad at stage III. (C) Section of gonad at stage IV. (D) Section of gonad at stage V. An arrowhead indicates a positive signal. Bar = 50 μm.