Tissue distribution of Foxl2 in three-spot wrasse tissues. Each bar represents mean ± standard error of mean, as determined by a real time quantitative polymerase chain reaction PCR assay. Values were normalized with a reference gene, ef1a. The transcript level is expressed relative to that of the brain sample, which represents the lowest detectable expression level. ND: transcripts were non-detectable. Numbers in parentheses indicated above the bars represent sample size.